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Understanding Fluorescence Microscopy

Want to Customize Your Life Sciences System?

Our Fluorescence Microscope, built entirely from components found in the Edmund Optics catalog, allows you to quickly customize to your application needs. The microscope, which is structured using TECHSPEC® Cage System components for unlimited adaptability, facilitates the easy exchange of light sources, objectives, and filters. In our demo, the microscope features a white light Lumencor SOLA Light Engine and Coherent® OBIS™ Lasers to allow for the excitation of almost any fluorophore in the visible spectrum. By incorporating a bandpass filter, one can achieve excitation efficiency similar to that of a diode-based laser.

Additionally, a Zaber ASR Motorized Microscope Stage can be added for faster scanning capabilities, which is ideal for larger samples that need to be completely inspected across the entire area. A camera and a wide variety of Mitutoyo objectives can be used to capture, save, and stitch together images once the scan is complete. The demo also utilizes TECHSPEC® Multi-Band Fluorescence Bandpass Filters, for enhanced fluorescence inspection.

Ready to build your own?

TECHSPEC® Optics
Description Stock Number
TECHSPEC® Fluorescent Filter Kit for Green Fluorescent Protein (GFP) #67-003
TECHSPEC® 506nm Dichroic Filter, 25.2 x 35.6mm #67-080
TECHSPEC® 525nm Bandpass Filter, 15nm Bandpass, OD6 Blocking, 25mm Dia #86-354
TECHSPEC® Achromatic Doublet Lens 25mm Dia. x 25mm FL, MgF2 Coating #65-553
TECHSPEC® Plastic Aspheric Lens, 25mm Diameter x 20mm FL, VIS Coating #66-014
TECHSPEC® Cage System
Description Stock Number
TECHSPEC® Cage Sphere with (5) 30mm Ports and (1) 43mm Port #85-624
TECHSPEC® 25mm Standard Cage Plate with ¼-20 for Post Mounting #85-633
TECHSPEC® Interchangeable Filter Holder #85-649
TECHSPEC® Filter Holder Plate #86-018
Objectives
Description Stock Number
2X Mitutoyo Plan Apo Infinity Corrected Long WD Objective #46-142
5X Mitutoyo Plan Apo Infinity Corrected Long WD Objective #46-143
7.5X Mitutoyo Plan Apo Infinity Corrected Long WD Objective #66-383
10X Mitutoyo Plan Apo Infinity Corrected Long WD Objective #46-144
20X Mitutoyo Plan Apo Infinity Corrected Long WD Objective #46-145
Mitutoyo Manual Turret #86-589
Illumination
Description Stock Number
Lumencor Sola SM Manual Operation Light Engine #88-434
Coherent OBIS™ 488nm LS 80mW Laser #88-024
Coherent OBIS™ 660nm LX 100mW Laser #87-468

In addition to stereo microscopes, check out our selection of infinity corrected, finite conjugate, or reflective objectives, eyepieces, or optical filters for microscopy setups.

De la biométrie au séquençage d'ADN en passant par le diagnostique in vitro, retrouvez des produits de qualité, un contenu technique et un savoir-faire d'une seule et même source.

Apprenez comment créer un montage de microscopie de fluorescence avec une illumination in-line et un filtrage pour optimiser le contraste et quantifier les émissions.

Suivez les instructions détaillées pour construire un microscope de fluorescence à l'aide des composants du Système de Cage Optique TECHSPEC®. (PDF 1.03 Mo)

Nous vous présentons des démos interactives et des nouveaux produits. En plus, vous avez la possibilité de discuter de vos projets avec notre équipe.

Besoin d'une offre ? Insérez un numéro de stock pour recevoir votre devis !

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